English Posts

Fluorescence quenching and Stern-Volmer equation

Abstract: in this article we describe some fluorescence quenching measures in some substances. The phenomenon of quenching occurs when some molecules absorb the energy of the fluorophores. This interesting phenomenon can be used in quantitative measurements for the determination of the quencher concentration and also, through the Stern-Volmer model, for …

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Arc Atomic Emission Spectroscopy

Abstract: in this article we describe the construction of a simple apparatus for spectroscopic analysis of atomic light emission from electric arc. The apparatus is based on a high voltage generator that produces an electric arc whose light emission is analyzed by a fiber optic spectrometer.

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an OPSL Laser

Abstract: in this post we describe the use of the Osram P530 laser. It is an optically pumped solid state laser (OPSL). The laser emits at 530 nm with power that can easily go up to 50-70 mW. It is a very interesting laser source for its low cost and the quality of the beam.

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Iodine Vapor Absorption

Abstract: in this post we describe the spectroscopic analysis of the absorption of iodine vapors. The maxima of the absorption are related to the transitions between the vibrational levels of the diatomic iodine molecule.

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Iodine Vapor Fluorescence

Abstract: in this article we describe the spectroscopic analysis of the fluorescence emission of iodine vapors excited by laser light at 532 nm. The maximums of the emission are related to the energy transitions between the vibrational levels of the diatomic iodine molecule.

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Polymer Analysis using Raman Spectroscopy

Abstract: in this article we describe the application of the Raman spectrometer (in backscattering configuration) to the analysis of plastic polymers with the aim of demonstrating the applicability of the tool to recognize the type of plastic. In the image above we show the Raman spectra of some plastic materials, …

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Backscattering Raman System

Abstract: in this article we describe the construction of a DIY Raman system, in backscattering configuration, based on a 532 nm DPSS laser and a B&W Tek surplus spectrometer.  The system is equipped with optical fiber and sharp-edge filters for blocking the radiation from excitation laser.

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Raman System DIY

Abstract: in this article we describe the construction of a DIY Raman system, based on a 532 nm DPSS laser and a B&W Tek surplus spectrometer. A 90° configuration was chosen for the collection of scattered light, particularly suitable for the analysis of liquid samples. The system is equipped with optical fiber and sharp-edge filters for blocking the radiation from excitation laser. The system has been used for acquisition of the Raman spectra of numerous organic and inorganic substances.

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Photosynthesis Pigments Spectroscopy

Abstract: in this post we describe the application of the SMA Thunder Optics spectrometer and the Spectragryph software to the spectroscopic analysis of the pigments responsible for the photosynthetic process, pigments found in all plant organisms (plants and algae).

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